Erythromycin (Ery_06) Aptamer Details
ID# 10033
Structure
Chemistry
DNA
Target
Erythromycin
Category
Other
Affinity (Kd)
20 ± 9 nM
Length
82
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdGpdApdApdTpdTpdCpdApdCpdGpdTpdCpdTpdCpdApdCpdTpdGpdGpdApdTpdTpdCpdApdCpdGpdCpdApdCpdGpdCpdCpdApdApdGpdGpdApdCpdTpdGpdCpdApdCpdTpdTpdApdApdGpdGpdTpdTpdApdGpdApdTpdApdGpdCpdCpdCpdCpdApdTpdGpdCpdApdGpdTpdGpdApdGpdTpdCpdApdGpdGpdApdTpdApdTpdCp-3'
Molecular Weight: 25274.4 g/mole
Extinction Coefficient: 798000
Binding Conditions / Buffer
1 mM CaCl2, 2.5 mM KCl, 1.5 mM KH2PO4, 0.5 mM MgCl2·6H2O, 137 mM NaCl, 8 mM Na2HPO4, pH 7.2
Binding Temp: Room temperature°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yepeng Du, Dan Liu, Min Wang, Fangke Guo, Jun Sheng Lin,
Preparation of DNA aptamer and development of lateral flow aptasensor combining recombinase polymerase amplification for detection of erythromycin, Biosensors and Bioelectronics,
Volume 181, 2021, 113157, ISSN 0956-5663, https://doi.org/10.1016/j.bios.2021.113157.
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