ErbB2 (SE15-8) Aptamer Details
ID# 7597
Structure
Chemistry
2'-F-RNA
Target
ErbB2 in Breast Cancer Cells
Category
Protein
Affinity (Kd)
3.49 nM
Length
34
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rAprGpfCpfCprGpfCprGprAprGprGprGprGprAprGprGprGprApfUprAprGprGprGpfUprAprGprGprGpfCprGpfCprGprGpfCpfUp-3'
Molecular Weight: 11239.85
Extinction Coefficient: 341100
Binding Conditions / Buffer
Binding buffer: 20 mM HEPES (pH 7.0), 150 mM NaCl, 1 mM MgCl2, 2 mM DTT, and 40 U RNase Inhibitor
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kim and Jeong. "In Vitro Selection of RNA Aptamer and Specific Targeting of ErbB2 in Breast Cancer Cells." Oligonucleotides, doi:10.1089/oli.2011.028.
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