Epithelial Cell Adhesion Molecule (EpCAM) SYL3C Aptamer Details

ID# 7980

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Structure

Chemistry

DNA

Target

Cancer Cells

Sequence (3-char table code)

G A C U/T

5'-dCpdApdCpdTpdApdCpdApdGpdApdGpdGpdTpdTpdGpdCpdGpdTpdCpdTpdGpdTpdCpdCpdCpdApdCpdGpdTpdTpdGpdTpdCpdApdTpdGpdGpdGpdGpdGpdGpdTpdTpdGpdGpdCpdCpdTpdGp-3'

Molecular Weight: 14838.6

Extinction Coefficient: 443700

Binding Conditions / Buffer

1 x PBS (pH-7.4) and 5 mM MgCl2

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Selection of DNA Aptamers against Epithelial Cell Adhesion Molecule for Cancer Cell Imaging and Circulating Tumor Cell Capture Yanling Song, Zhi Zhu, Yuan An, Weiting Zhang, Huimin Zhang, Dan Liu, Chundong Yu, Wei Duan, and Chaoyong James Yang Analytical Chemistry 2013 85 (8), 4141-4149 DOI: 10.1021/ac400366b

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