Epithelial cell adhesion molecule (EpCAM) (EpDT3) Aptamer Details
ID# 7739
Structure
Chemistry
2'-F-RNA
Target
Cancer stem cell marker epithelial cell adhesion molecule
Category
Protein
Affinity (Kd)
54.5 nM
Length
20
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGpfCprGprApfCpfUprGprGpfUpfUp rApfCpfCpfCprGprGpfUpfCprGpdTi-3'
Molecular Weight: 6392.85
Extinction Coefficient: 188200
Binding Conditions / Buffer
Assay Buffer: DPBS with 5 mM MgCl2, 0.1 mg/ml tRNA, 0.1 mg/ml salmon sperm DNA, 02% [w/v] sodium azide, and 5% FCS. The final concentration of MgCl2 was 2.5 mM.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
S Shigdar et al. RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule. Cancer Sci 102(2011): 991-998.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
