EP23 Aptamer Details

ID# 8104

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Structure

Chemistry

RNA

Target

Epithelial cellular activating molecule (EpCAM)

Sequence (3-char table code)

G A C U/T

5'-rAprCprGprUprAprUprCprCprCprUprUprUprUprCprGprCprGprUprAp-3'

Molecular Weight: 5631.6

Extinction Coefficient: 165300

Binding Conditions / Buffer

PBS containing 5 mM MgCl2

Binding Temp: 25 and 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Bell DR, Weber JK, Yin W, Huynh T, Duan W, Zhou R. In silico design and validation of high-affinity RNA aptamers targeting epithelial cellular adhesion molecule dimers. Proc Natl Acad Sci U S A. 2020 Apr 14;117(15):8486-8493. doi: 10.1073/pnas.1913242117. Epub 2020 Mar 31. Erratum in: Proc Natl Acad Sci U S A. 2021 Feb 16;118(7): PMID: 32234785; PMCID: PMC7165443.

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