Enthalpy Driven Aptamer SYL-H2C Aptamer Details

ID# 8044

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Structure

Chemistry

DNA

Target

EpCAM

Sequence (3-char table code)

G A C U/T

5' dGpdApdGpdCpdTpdCpdGpdGpdGpdGpdTpdTpdTpdGpdGpdGpdGpdGpdTpdTpdCpdGpdGpdGpdGpdTpdCpdGpdGpdTpdTpdCpdGpdGpdTpdTpdTpdCpdTpdTp 3'

Molecular Weight: 12500.12

Extinction Coefficient: 403290

Binding Conditions / Buffer

PBS, pH=7.4 (including 136.8 mM NaCl, 10.1 mM Na2HPO4, 2.7 mM KCl, 1.8 mM KH2PO4, 0.55 mM MgCl2)

Binding Temp: 37°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Huang, M., Song, J., Huang, P., Chen, X., Wang, W., Zhu, Z., Song, Y., & Yang, C. (2019). Molecular Crowding Evolution for Enabling Discovery of Enthalpy-Driven Aptamers for Robust Biomedical Applications. Analytical Chemistry, 91(16), 10879–10886. https://doi.org/10.1021/acs.analchem.9b02697

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