Enterotoxin B (APT SEB1) Aptamer Details
ID# 7685
Structure
Chemistry
DNA
Target
Staphylococcus aureus Enterotoxin B
Category
Protein
Affinity (Kd)
N/A nM
Length
78
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdTpdApdTpdTpdGpdApdGpdGpdGpdTpdCpdGpdCpdApdTpdCpdCpdApdCpdTpdGpdGpdTpdCpdGpdTpdTpdGpdTpdTpdGpdTpdCpdTpdGpdTpdTpdGpdTpdCpdTpdGpdTpdTpdApdTpdGpdTpdTpdGpdTpdTpdTpdCpdGpdTpdGpdApdTpdGpdGpdCpdTpdCpdTpdApdApdCpdTpdCpdTpdCpdCpdTpdCpdTp-3'
Molecular Weight: 24022.61
Extinction Coefficient: 707600
Binding Conditions / Buffer
Binding buffer: 10 mM phosphate buffer, 2.7 mM KCl, 140 mM NaCl, 0.05% Tween, pH 7.4,
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
DeGrasse, J. (2012). A single-stranded DNA aptamer that selectively binds to Staphylococcus aureus enterotoxin b. PloS One, 7(3): e33410. doi: 10.1371/journal.pone.0033410
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