Enterotoxigenic Escherichia coli (E. coli) fimbriae protein K88 (clone 37) Aptamer Details

ID# 7924

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Structure

Chemistry

DNA

Target

K88

Sequence (3-char table code)

G A C U/T

5'-dGpdApdGpdApdCpdCpdGpdTpdApdCpdCpdApdTpdCpdTpdGpdTpdTpdCpdGpdTpdGpdGpdApdApdGpdCpdGpdCpdTpdTpdTpdGpdCpdTpdCpdGpdTpdCpdCpdApdTpdTpdApdGpdCpdCpdTpdTpdGpdTpdGpdCpdTpdCpdGpdTpdGpdCp-3'

Molecular Weight: 18431.95

Extinction Coefficient: 545200

Binding Conditions / Buffer

20 mmol/L HEPES (pH 7.35), 1 mmol/L MgCl2; 1 mmol/L CaCl2; and 120 mmol/L KCl

Binding Temp: 25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Li, H. et al. Aptamer selection for the detection of Escherichia coli K88. 2011. Canadian Journal of Microbiology 57.6: 453-9.

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