Endothelial Cells (P36) Aptamer Details
ID# 7594
Structure
Chemistry
Peptide
Target
Endothelial Cells
Category
Cells
Affinity (Kd)
2.2 nM
Length
12
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
NH2-SerGlnArgTrpThrAlaLeuTrpGlnTrpIleGly-COOH
Molecular Weight: 1667.95
Extinction Coefficient:
Binding Conditions / Buffer
Binding Buffer (1 mM glucose, 1% BSA, 10 mM MgSO4) Selection Buffer (0.1% BSA, 1 mM CaCl2, and 10 mM MgCl2)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Herrmann et al. "Selection of cell specific peptides in a rat carotid injury model using a random peptide-presenting bacterial library." Bichemica et Biophysica Acta, 1472(1999): 529-536.
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