EF508 Aptamer Details
ID# 8096
Structure
Chemistry
DNA
Target
Enterococcus faecalis bacteria
Category
Other
Affinity (Kd)
37 nM
Length
86
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dTpdApdGpdGpdGpdApdApdGpdApdGpdApdApdGpdGpdApdCpdApdTpdApdTpdGpdApdTpdApdCpdTpdGpdGpdCpdCpdTpdTpdGpdApdCpdApdCpdCpdCpdTpdGpdTpdTpdGpdTpdGpdGpdCpdTpdTpdGpdApdTpdGpdApdCpdApdApdTpdApdApdCpdApdTpdTpdGpdApdCpdTpdApdGpdTpdApdCpdApdTpdGpdApdCpdCpdApdCpdTpdTpdGpdAp-3’
Molecular Weight: 26627.3
Extinction Coefficient: 853800
Binding Conditions / Buffer
10 mM of Tris–EDTA bufer (pH 8.5)
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kolm C, Cervenka I, Aschl UJ, et al. DNA aptamers against bacterial cells can be efficiently selected by a SELEX process using state-of-the art qPCR and ultra-deep sequencing. Sci Rep. 2020;10(1):20917. Published 2020 Dec 1. doi:10.1038/s41598-020-77221-9
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