EAg3-Py Aptamer Details
ID# 8034
Structure
Chemistry
DNA
Target
Hepatitis B - E Antigen (HBeAg)
Category
Protein
Affinity (Kd)
0.4 nM
Length
48
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdTpdTpdTpdTpdTpdTpdTpdGpdGpdGpdCpdGpdApdApdGpdApdCpdCpdGpdGpdGpdApdCpdGpdGpdGpdApdGpdGpdApdApdApdGpdApdGpdApdTpdGpdTpdTpdTpdGpdGpdTpdTpdTpdTp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
1× PBS buffer (10 mM sodium
phosphate, 137 mM NaCl, and 4.5 mM KCl, pH 7.4)
7 mM MgCl2 added after refolding
Validated against patent samples in blood serum.
Binding Temp: 37°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Aptamer Binding Assay for the E Antigen of Hepatitis B Using Modified Aptamers with G-Quadruplex Structures
Yanming Liu, Connie Le, D. Lorne Tyrrell, X. Chris Le, and Xing-Fang Li
Analytical Chemistry 2020 92 (9), 6495-6501
DOI: 10.1021/acs.analchem.9b05740
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