E. Coli Release Factor 1 (Clone II-1) Aptamer Details
ID# 7528
Structure
Chemistry
RNA
Target
E. Coli Release Factor 1
Category
Small Organic
Affinity (Kd)
30 nM
Length
85
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprAprCprCprGprAprGprAprAprGprUprUprAprCprCprCprUprGprUprAprAprUprCprUprUprAprGprGprAprUprGprAprAprUprCprGprCprAprUprGprCprUprCprUprAprGprCprGprAprCprCprUprUprUprUprCprGprGprCprUprUprCprGprGprCprGprUprAprCprGprCprAprCprAprUprCprGprCprAprGprCprAprAprCp-3'
Molecular Weight: 27219.37
Extinction Coefficient: 824600
Binding Conditions / Buffer
Elution Buffer (250 mM imidazole)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Sando, et al. "In vitro selection of RNA aptamer against Escherichia coli release factor 1." Bioorganic & Medicinal Chemistry Letters, 17(2007): 1216-1220.
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