Aptagen

E. Coli 5S RNA (Helix 89 RNA) Aptamer Details

ID# 7516
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Structure
E. Coli 5S RNA (Helix 89 RNA) structure
Chemistry
RNA
Target
E. Coli 5S RNA
Category
Small Organic
Affinity (Kd)
3 µM
Length
37
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-rGprGprUprGprAprUprAprCprCprGprCprCprCprAprAprGprAprGprUprUprCprAprUprAprUprCprGprAprCprGprGprCprGprGprUprGprUp-3'
Molecular Weight: 11910.22
Extinction Coefficient: 366600

Binding Conditions / Buffer

Binding buffer: 30 mM Trisƒ??acetate(pH 7.5), 60 mM magnesium acetate, 120 mM potassium acetate, and 120 mM ammonium acetate.
Binding Temp: RT°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Ko, J. et al. "Identification of a structural motif of 23S rRNA interacting with 5S rRNA." FEBS Letters, 508 (2001): 300-304.
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