Dopamine (dopa2/c.4) Aptamer Details

ID# 7766

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Structure

Chemistry

RNA

Target

Dopamine

Sequence (3-char table code)

G A C U/T

5'-rGprGprGp rAprAprUp rUprCprCp rGprCprGp rUprGprUp rGprCprGp rCprCprGp rCprGprGp rAprAprGp rAprGprGp rGprAprAp rUprAprUp rAprGprAp rGprGprCp rCprAprGp rCprAprCp rAprUprAp rGprUprGp rAprGprGp rCprCprCp rUprCprCp rUprCprCp rCp-3'

Molecular Weight: 21676.14

Extinction Coefficient: 652400

Binding Conditions / Buffer

Assay Buffer: 10 mM PBS, pH 7.2, 5mM MgCl2

Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Sequence from C Mannironi, et al. "In Vitro Selection of Dopamine RNA Ligands." Biochemistry, 36 (1997): 9726-973. Enzyme-linked assay from H Park and I R Paeng. "Development of direct competitive enzyme-linked aptamer assay for determination of dopamine in serum." Analytica Chimica Acta, 685 (2011): 65-73.

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