Dopamine (dopa1.30/c.30) Aptamer Details
ID# 7767
Structure
Chemistry
RNA
Target
Dopamine
Category
Small Organic
Affinity (Kd)
N/A nM
Length
58
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprUprCp rUprCprUp rGprUprGp rUprGprCp rGprCprCp rAprGprAp rGprAprAp rCprAprCp rUprGprGp rGprGprCp rAprGprAp rUprAprUp rGprGprGp rCprCprAp rGprCprAp rCprAprGp rAprAprUp rGp rAprGprGp rCprCprCp-3'
Molecular Weight: 18784.41
Extinction Coefficient: 567600
Binding Conditions / Buffer
Column Buffer: 50 mM Tris-HCl (pH 7.4), 5 mM MgCl2, and 0.5 M or 0.15 M NaCl
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Mannironi, C., et al. "In Vitro Selection of Dopamine RNA Ligands." Biochemistry, 36 (1997): 9726-9734.
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