Dopamine (DNA version of dopa1.30/c.30) Aptamer Details
ID# 7768
Structure
Chemistry
DNA
Target
Dopamine
Category
Small Organic
Affinity (Kd)
0.7 µM
Length
58
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdTpdCp dTpdCpdTp dGpdTpdGp dTpdGpdCp dGpdCpdCp dApdGpdAp dGpdApdAp dCpdApdCp dTpdGpdGp dGpdGpdCp dApdGpdAp dTpdApdTp dGpdGpdGp dCpdCpdAp dGpdCpdAp dCpdApdGp dApdApdTp dGp dApdGpdGp dCpdCpdCp-3'
Molecular Weight: 17982.68
Extinction Coefficient: 561000
Binding Conditions / Buffer
Buffer: 50 mM Tris-HCl (pH 7.4), 5 mM MgCl2, and 0.5 M
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
R Walsh and M DeRosa. Retention of function in the DNA homolog of the RNA dopamine aptamer. Biochem. Biophys. Res. Commun. 388(2009): 732-735.
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