dNCL#T1 for Nucleolin Aptamer Details

ID# 8958

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Structure

Chemistry

DNA

Target

Nucleolin

Sequence (3-char table code)

G A C U/T

dTpdGpdGpdTpdGpdGpdTpdGpdGpdTpdGpdGpdTpdTpdGpdTpdGpdGpdTpdGpdGpdTpdGpdGpdTpdGpdGp

Molecular Weight: 8576.5 Da

Extinction Coefficient: 258300

Binding Conditions / Buffer

Incubated in 10 mM Tris−HCl, 10 mM CuSO4, 10 mM sodium erythorbate solution at 37 C for 6 hours.

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Inducible Degradation of Oncogenic Nucleolin Using an Aptamer-Based PROTAC Miao Chen, Ping Zhou, Yun Kong, Jingrui Li, Yan Li, Yao Zhang, Jie Ran, Jun Zhou, Yan Chen, and Songbo Xie Journal of Medicinal Chemistry 2023 66 (2), 1339-1348 DOI: 10.1021/acs.jmedchem.2c01557

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