DNAzymeMB (Part of Lysozyme Aptasensor) Aptamer Details
ID# 7581
Structure
Chemistry
DNA
Target
N/A: See additional comments.
Category
Protein
Affinity (Kd)
0.1 fM
Length
28
Aptazyme
Yes
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdGpdGpdApdCpdGpdGpdGpdCpdTpdApdApdGpdTpdApdApdCpdTpdGpdTpdGpdGpdApdGpdGpdGpdTp-3'
Molecular Weight: 8798.78
Extinction Coefficient: 285700
Binding Conditions / Buffer
NEBuffer 2(10X): 50 mM NaCl, 10 mM Tris-HCL, 10 mM MgCl2 1 mM dithiothreitol, pH 7.9.
Detection Buffer (total volume): 25 mM HEPES, 20 mM KCL, 200 mM NaCl, 0.05% Triton X-100 and 1% DMSO; pH 7.4) with 0.5 µM hemin.
Consult ref. for procedure.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Fu et al. "An ultrasensitive peroxidase DNAzyme-associated aptasensor that utilizes a target-triggered enzymatic signal amplification strategy." Chemical Communications, 47(2011): 9876-9878.
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