Diversely Functionalized Human alpha-Thrombin Aptamer Aptamer Details

ID# 8028

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Structure

Chemistry

DNA

Target

Human alpha-Thrombin

Sequence (3-char table code)

G A C U/T

GGATCCGAGCTCCACGTG ACTGC ATCTA ATTCC AACCT ATCCA ATCCT ATTCG ATGCT TGCGACGGCAGGCGAATC

Molecular Weight: 23.338 kDa

Extinction Coefficient:

Binding Conditions / Buffer

20 mM Tris-HCl pH 7, 140 mM NaCl, 5mM KCl, 1 mM MgCl2, 1 mM CaCl2

Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

DOI: 10.1021/jacs.7b07241 J. Am. Chem. Soc. 2017, 139, 13977−13980

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