Digoxin (Truncated D1) Aptamer Details
ID# 7770
Structure
Chemistry
DNA
Target
Digoxin
Category
Protein
Affinity (Kd)
0.05 nM
Length
50
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdCpdGpdApdGpdGpdGpdCpdGp dGpdTpdGpdTpdCpdCpdApdApdCpdAp dGpdCpdGpdGpdTpdTpdTpdTpdTpdTp dCpdApdCpdGpdApdGpdGpdApdGpdGp dTpdTpdGpdGpdCpdGpdGpdTpdGpdGp-3'
Molecular Weight: 15635.16
Extinction Coefficient: 482000
Binding Conditions / Buffer
Selection Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween 20.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Z Kiani et al. In vitro selection and characterization of deoxyribonucleic acid aptamers for digoxin. Analytica Chimica Acta 748(2012): 67-72.
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