Aptagen

Di(2-ethylhexyl) phthalate (DEHP) (Aptamer 31) Aptamer Details

ID# 10059
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Structure
Di(2-ethylhexyl) phthalate (DEHP) (Aptamer 31) structure
Chemistry
DNA
Target
Di(2-ethylhexyl) phthalate (DEHP)
Category
Small Organic
Affinity (Kd)
2.26 ± 0.06 nM
Length
58
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dApdCpdGpdCpdApdTpdApdGpdGpdGpdTpdGpdCpdGpdApdCpdCpdApdCpdApdTpdApdCpdGpdCpdCpdCpdCpdApdTpdGpdTpdApdTpdGpdTpdCpdCpdCpdTpdTpdGpdGpdTpdTpdGpdTpdGpdCpdCpdCpdTpdApdTpdGpdCpdGpdTp-3'
Molecular Weight: 17776.5 g/mole
Extinction Coefficient: 536100

Binding Conditions / Buffer

DPBS buffer (0.1 g CaCl2, 0.2 g KCl, 0.2 g KH2PO4, 0.1 g MgCl2.6H2O, 8 g NaCl, and 1.15 g Na2HPO4; 1 L, pH 7.5)
Binding Temp: 25°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Lu, Q.; Liu, X.; Hou, J.; Yuan, Q.; Li, Y.; Chen, S. Selection of Aptamers Specific for DEHP Based on ssDNA Library Immobilized SELEX and Development of Electrochemical Impedance Spectroscopy Aptasensor. Molecules 2020, 25, 747. https://doi.org/10.3390/molecules25030747
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