DE3A Aptamer Details
ID# 8020
Structure
Chemistry
2'-F-RNA
Target
Sickle Hemoglobin
Category
Protein
Affinity (Kd)
1.68 µM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGpfCpfCprGprApfUpfUprAprGprAprApfCpfUprGprGprGpfCpfUprGprAprGprGpfCprGpfUpfUpfCpfUprGpfCprApfUpfUpfUpfCprGprGpfUprGprApfUpfCprAprGprApfCprGprApfCpfUpfCprGpfCpfUprGprAprGprGprApfUpfCpfCprGprAprGprAp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
20 mM HEPES pH 7.4, 150 mM NaCl, 2 mM CaCl2,
0.01% BSA
Binding Temp: 20°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Purvis, S. H., Keefer, J. R., Fortenberry, Y. M., Barron-Casella, E. A., & Casella, J. F. (2017). Identification of Aptamers That Bind to Sickle Hemoglobin and Inhibit Its Polymerization. Nucleic Acid Therapeutics, 27(6), 354–364. doi:10.1089/nat.2016.0646
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