D-Tryptophan (MF-10) Aptamer Details
ID# 7515
Structure
Chemistry
RNA
Target
D-Tryptophan
Category
Small Organic
Affinity (Kd)
18 nM
Length
153
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rAprGprUprAprAprUprAprCprGprAprCprUprCprAprCprUprAprUprAprGprGprGprAprGprAprAprUprUprCprCprGprAprCprCprAprGprAprAprGprUprUprGprGprCprGprUprUprGprGprCprAprUprGprAprCprGprCprGprGprGprGprAprAprUprCprGprGprGprUprGprCprAprUprCprGprAprUprGprAprCprUprAprCprUprCprCprUprGprGprGprCprCprCprAprCprGprUprCprUprGprUprUprGprUprUprGprAprCprGprUprCprAprCprAprGprCprUprUprGprAprUprUprUprAprGprGprAprUprAprGprCprGprCprUprUprGprGprGprCprAprGprUprCprGprUprGprCprAprGprUprGprGprAp-3'
Molecular Weight: 49445.7
Extinction Coefficient: 1515800
Binding Conditions / Buffer
Column Buffer (4 mM Mg2+, 250 mM NaCl, 50 mM Tris C1 (pH 7.6)) Binding Buffer is (250 mM NaCl, 4 mM MgCl2, 50 mM Tris (pH 7.6)) Elution Buffer (20 mM EDTA (pH 8.0))
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Famulok and Szostak. "Stereospecific Recognition of Tryptophan Agarose by in Vitro Selected RNA." Journal of American Chemical Society, 114 (1992): 3990
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