Cyclic Adenosine Monophosphate (AR4) Aptamer Details

ID# 7591

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Structure

Chemistry

RNA

Target

cAMP

Sequence (3-char table code)

G A C U/T

5'-rGprGprAprUprAprAprUprAprGprCprCprGprUprAprGprGprUprUprGprCprGprAprAprAprGprCprGprAprCprCprCprUprGprAprUprGprAprGprCprCprUp rGprUprGp rGprAprAp rAprCprAp rGprAprCp rGprUprGp rGprCprAp rCprAprUp rGprAprCp rUprAprCp rGprUprCprGprAprAprAprCprGprGprUprAprGprCprGprAprGprAprGprCprUprCp-3'

Molecular Weight: 29204.74

Extinction Coefficient: 905300

Binding Conditions / Buffer

Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl2 2X Gel Loading Buffer (equal volume used to quench reaction): 87 mM Tris base, 89 mM boric acid, 20% sucrose, 0.05% bromophenol blue, 0.05% xylene cyanol, 0.1% sodium dodecyl sulfate (SDS), 7.3 M urea, 1 mM EDTA. Additional 40 mM EDTA added.

Binding Temp: 23°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Seetharaman et al. "Immbolized RNA switches for the analysis of complex chemical and biological mixtures." Nature Biotechnology, 19(2001): 336-341.

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