Cyanocobalamin (35-mer) Aptamer Details
ID# 7513
Structure
Chemistry
RNA
Target
Cyanocobalamin
Category
Small Organic
Affinity (Kd)
88 nM
Length
35
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rCprCprGprGprUprGprCprGprCprAprUprAprAprCprCprAprCprCprUprCprAprGprUprGprCprGprAprGprCprAprAprGprGprAprAp-3'
Molecular Weight: 11263.9
Extinction Coefficient: 341600
Binding Conditions / Buffer
Column Buffers (1 M LiC1, 5 mM MgC12, and 25 mM HEPES (pH 7.4) or 1 M NaCl, 5 mM MgC12, and 25 mM HEPES (pH 7.4))
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lorsch and Szostak. "In vitro selection of RNA aptamers specific for cyanocobalamin." Biochemistry, 33 (1994): 973-982.
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