CTBA4T-B1 Aptamer Details
ID# 8088
Structure
Chemistry
DNA
Target
Thrombin
Category
Peptide
Affinity (Kd)
19 pM
Length
55
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdCpdTpdCpdGpdApdGpdTpdCpdApdTpdApdGpdGpdGpdGpdGpdCpdGpdCpdGpdApdApdCpdApdTpdApdCpdGpdCpdGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGpdCpdTpdGpdApdCpdTpdCpdGpdTp-3'
Molecular Weight: 17110.1
Extinction Coefficient: 525200
Binding Conditions / Buffer
Binding buffer (1× PBSMT, pH 7.2) containing 137 mM NaCl, 2.68 mM KCl, 8.1 mM Na2HPO4, 1.76 mM KH2PO4, 1 mM MgCl2, and 0.025% Tween-20.
Binding Temp: 95, 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Mao, Yu et al. “Evolution of a highly functional circular DNA aptamer in serum.” Nucleic acids research vol. 48,19 (2020): 10680-10690. doi:10.1093/nar/gkaa800
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