CTBA4 Aptamer Details
ID# 8059
Structure
Chemistry
DNA
Target
Thrombin
Category
Protein
Affinity (Kd)
0.15 nM
Length
87
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdCp dTpdCpdGp dApdCpdTp dApdGpdTp dCpdApdTp dApdGpdGp dGpdGpdGp dCpdGpdCp dGpdApdAp dCpdApdTp dApdCpdGp dCpdGpdGp dTpdTpdGp dGpdTpdGp dTpdGpdGp dTpdTpdGp dGpdCpdTp dGpdApdCp dApdApdTp dApdCpdTp dCpdGpdTp dTpdTpdTp dTpdGpdGp dTpdGpdTp dCpdTpdCp dGpdGpdAp dTp-3'
Molecular Weight: 24520.8
Extinction Coefficient: 751900
Binding Conditions / Buffer
1× PBSMT, pH 7.2, contains 137 mM NaCl,
2.68 mM KCl, 8.1 mM Na2HPO4, 1.76 mM KH2PO4, 1
mM MgCl2, and 0.025% Tween-20
Binding Temp: 20°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yu Mao, Jimmy Gu, Dingran Chang, Lei Wang, Lili Yao, Qihui Ma, Zhaofeng Luo, Hao Qu, Yingfu Li, Lei Zheng, Evolution of a highly functional circular DNA aptamer in serum, Nucleic Acids Research, Volume 48, Issue 19, 4 November 2020, Pages 10680–10690
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