Aptagen

Crude Extracellular Mixture of E. coli – trans Aptamer Details

ID# 7948
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Structure
Crude Extracellular Mixture of E. coli – trans structure
Chemistry
DNA
Target
Crude Extracellular Matrix of E. coli
Category
Protein
Affinity (Kd)
N/A nM
Length
210
Aptazyme
Yes

Sequence (3-char table code)

G A C U/T
5'-dGpdApdTpdGpdTpdGpdCpdGpdTpdTpdGpdTpdCpdGpdApdGpdApdCpdCpdTpdGpdCpdGpdApdCpdCpdGpdGpdApdApdCpdApdCpdTpdApdCpdApdCpdTpdGpdTpdGpdTpdGpdGpdGpdGpdApdTpdGpdGpdApdTpdTpdTpdCpdTpdTpdTpdApdCpdApdGpdTpdTpdGpdTpdGpdTpdGp-3'
Molecular Weight: 21729.14
Extinction Coefficient: 667800

Binding Conditions / Buffer

Selection Buffer (1 mM HEPES (pH 7.4), 150 mM NaCl, 15 mM MgCl2, and 0.01% Tween 20), 60 min @ r.t.
Binding Temp: 23°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Aguirre et al. "A Sensitive DNA Enzyme-Based Fluorescent Assay for Bacterial Detection" Biomolecules, 3 (2013): 563-577.
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