Crude Extracellular Mixture of E. coli – cis Aptamer Details
ID# 7647
Structure
Chemistry
DNA
Target
Crude Extracellular Mixture (CEM) of E. coli
Category
Protein
Affinity (Kd)
N/A nM
Length
125
Aptazyme
Yes
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdApdCpdGpdGpdApdTpdCpdCpdTpdGpdApdCpdApdApdGpdGpdApdTpdGpdTpdGpdTpdGpdCpdGpdTpdTpdGpdTpdCpdGpdApdGpdApdCpdCpdTpdGpdCpdGpdApdCpdCpdGpdGpdApdApdCpdApdCpdTpdApdCpdApdCpdTpdGpdTpdGpdTpdGpdGpdGpdApdTpdGpdGpdApdTpdTpdTpdCpdTpdTpdTpdApdCpdApdGpdTpdTpdGpdTpdGpdTpdGpdCpdApdGpdCpdTpdCpdCpdGpdTpdCpdCpdGpdApdCpdTpdCpdTpdTpdCpdCpdTpdApdGpdCp-{Internal/Fluorescein-dT}-rAp-{Internal/Dabcyl-dT}-dGpdGpdTpdTpdCpdGpdApdTpdCpdApdApdGpdAp-3'
Molecular Weight: 38623.03
Extinction Coefficient: 1183600
Binding Conditions / Buffer
Selection Buffer (50 mm HEPES (pH 7.5), 150 mM NaCl, 15 mM MgCl2, and 0.01% Tween 20), r.t.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ali et al. "Fluorogenic DNAzyme Probes as Bacterial Indicators." Angewandte Chemie International Edition, 50 (2011): 3751-3754.
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