Aptagen

CoV2-RBD-4C Aptamer Details

ID# 8161
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Structure
CoV2-RBD-4C structure
Chemistry
DNA
Target
SARS-CoV-2 S protein / SARS-CoV-2 RBD
Category
Protein
Affinity (Kd)
13.6 nM
Length
276
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dApdTpdCpdCpdApdGpdApdGpdTpdGpdApdCpdGpdCpdApdGpdCpdApdTpdTpdTpdCpdApdTpdCpdGpdGpdGpdTpdCpdCpdApdApdApdApdGpdGpdGpdGpdCpdTpdGpdCpdTpdCpdGpdGpdGpdApdTpdTpdGpdCpdGpdGpdApdTpdApdTpdGpdGpdApdCpdApdCpdGpdTp-3'
Molecular Weight:
Extinction Coefficient:

Binding Conditions / Buffer

Buffer, Binding - PBS with 0.55 mM MgCl2
Binding Temp: Annealed at 95 °C, after which it was immediately cooled onice for 5 min and then at 25 °C for 10 min.

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Song, Y., Song, J., Wei, X., Huang, M., Sun, M., Zhu, L., Lin, B., Shen, H., Zhu, Z., and C. Yang. 2020. Discovery of Aptamers Targeting the Receptor-Binding Domain of the SARS-CoV‑2 Spike Glycoprotein. Analytical Chemistry 2020, 92, 9895−9900. https://dx.doi.org/10.1021/acs.analchem.0c01394
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