CoV RBD Protein (Linear Aptamer) Aptamer Details
ID# 8431
Structure
Chemistry
DNA
Target
CoV Spike Protein
Category
Protein
Affinity (Kd)
10.9 µM
Length
50
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdCpdGpdCpdGpdCpdGpdApdApdApdGpdTpdGpdApdCpdApdApdTpdTpdGpdGpdGpdCpdCpdTpdCpdGpdGpdCpdTpdGpdTpdGpdApdTpdTpdGpdTpdTpdCpdGpdApdGpdGpdGpdApdGpdApdCp-3'
Molecular Weight: 16500
Extinction Coefficient:
Binding Conditions / Buffer
Buffer: 1 ml of 1× SELEX buffer (20 mM Tris-HCl (pH:7.4), 2 mM
CaCl2, 5 mM KCl, 100 mM NaCl, 1 mM MgCl2).
Binding Temp: 23°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Simple in-vitro single stranded linear and circular DNA preparation and validation via SELEX using phosphor-derived modifications
Seyed Vahid Hamidi, Jonathan Perreault
bioRxiv 2023.02.11.528153; doi: https://doi.org/10.1101/2023.02.11.528153
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