Colicin E3 (F1-1) Aptamer Details
ID# 7490
Structure
Chemistry
RNA
Target
Colicin E3
Category
Protein
Affinity (Kd)
2 nM
Length
121
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprAprUprUprCprCprGprAprCprCprAprGprAprAprGprCprUprUprAprCprUprGprUprCprCprUprCprCprCprUprUprCprAprGprAprGprAprGprCprGprCprGprGprGprAprCprCprCprUprUprAprAprCprUprUprGprGprGprGprCprCprCprAprCprGprAprAprCprAprGprCprUprUprCprAprGprUprUprCprCprGprUprCprUprCprGprGprCprGprUprCprAprUprAprUprGprUprGprCprGprUprCprUprAprCprAprUprGprGprAprUprCprCprUprCprAp-3'
Molecular Weight: 38734.22
Extinction Coefficient: 1159200
Binding Conditions / Buffer
Binding Buffer (10 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 30 mM NH4Cl)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Hirao, I., et al. "In Vitro Selection of RNA Aptamers that Bind to Colicin E3 and Structurally Resemble the Decoding Site of 16S Ribosomal RNA." Biochemistry, 43 (2004): 3214-3221.
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