Cocaine (MNS-4.1) Aptamer Details
ID# 7783
Structure
Chemistry
DNA
Target
Cocaine
Category
Small Organic
Affinity (Kd)
0.4 µM
Length
38
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdApdGpdApdCpdApdApdGpdGpdApdApdApdApdTpdCpdCpdTpdTpdCpdApdApdTpdGpdApdApdGpdTpdGpdGpdGpdTpdCpdGpdApdCpdAp-3'
Molecular Weight: 11833.78
Extinction Coefficient: 392900
Binding Conditions / Buffer
Phosphate buffer: 20 mM, pH7, 200 mM NaCl.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
M Stojanovic, P de Prada, and D Landry. Aptamer-based folding fluorescent sensor for cocaine. J. Am. Chem. Soc. 123(2001): 4928-4931.
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