CN-ES1 (17β-Estradiol) Aptamer Details
ID# 9657
Structure
Chemistry
DNA
Target
17β-Estradiol
Category
Small Organic
Affinity (Kd)
83 ± 25 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dApdCpdGpdApdCpdGpdGpdTpdGpdGpdCpdApdTpdGpdApdApdCpdApdTpdApdApdGpdTpdTpdCpdCpdCpdCpdCpdTpdTpdGpdApdApdApdGpdTpdCpdGpdTp
Molecular Weight: 12305 g/mole
Extinction Coefficient: 389000
Binding Conditions / Buffer
SELEX Buffer: 20 mM Tris, pH 7.4, 500 mM NaCl, 10 mM MgCl2.
Sensing Buffer: 20 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM MgCl2
Binding Temp: Room Temperature°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Capture-SELEX of DNA Aptamers for Estradiol Specifically and Estrogenic Compounds Collectively Chenqi Niu, Chong Zhang, and Juewen Liu Environmental Science & Technology 2022 56 (24), 17702-17711 DOI: 10.1021/acs.est.2c05808
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