Clone 488 Aptamer Details
ID# 7915
Structure
Chemistry
RNA
Target
SelB
Category
Protein
Affinity (Kd)
50 mM potassium phosphate, pH 7.0, 5 mM Mg(OAc)2, 0.1 mM EDTA, 1 mM DTT, 0.5 mM GTP,0.02% Tween 20, 400 U/mL RNAsin
Length
67
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dGpdCpdGpdCpdTpdApdApdGpdTpdCpdCpdTpdCpdGpdCpdTpdCpdApdGpdCpdCpdCpdApUdApdApdGpUUdGpUdCpdCpdCpdApdApdGpUdCpUUdGpdGpdGpdCpdGpdCpdApdApdApUdApdCpdApUdCpdCpdCpdApdCpdGpdCpdGpdCpdGpdApdCpdTpdCpdGpdGpdApdTpdCpdCpdGp
Molecular Weight: 20508.24
Extinction Coefficient: 621000
Binding Conditions / Buffer
37°C
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Klug, S. J. et al. "In vitro selection of RNA aptamers that bind special elongation factor SelB, a protein with multiple RNA-binding sites, reveals one major interaction domain at the carboxyl terminus." RNA, 1995 5: 1180 - 1190.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
