Clone 2 Aptamer Details

ID# 7902

Back to Search Results

Structure

Chemistry

2'-F-RNA

Target

Protein E (PE)

Sequence (3-char table code)

G A C U/T

dCpdGpdApdCpUdGpdCpdApdGpdApdGpdCpUUdGpdCpUdApdCpdGpUUdGpUdApdApUUdCpdApdApdApdApdCpdApdApdApdGpdGpUUUdCpdCpUdGpdGpdApdCpUdCpdCpdCpdCpdGpdCpdApdGpdGpUdCpUUdGpUdGpdApdApUdGpdGpUdApdCpdCpdGpdApdGpdCpUdCpdGpdApdApUUdCpdCpdCp

Molecular Weight: 20369.18

Extinction Coefficient: 643700

Binding Conditions / Buffer

165 ug (~5 nmol) purified truncated His-tagged PE immobilized on Niƒ??NTA agarose (Qiagen) in 1.6 mL PBSM (PBS with 1 mM MgCl2)

Binding Temp: 23°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Barfod, Anders et al. "In Vitro Selection of RNA Aptamers Directed Against Protein E: A Haemophilus influenzae Adhesin." Mol Biotech, 2014: 1-12.

Request a Quote Have your aptamer oligo synthesized by Aptagen.