Clone 1 Aptamer Details
ID# 7901
Structure
Chemistry
2'-F-RNA
Target
Protein E (PE)
Category
Protein
Affinity (Kd)
165 ug (~5 nmol) purified truncated His-tagged PE immobilized on Niƒ??NTA agarose (Qiagen) in 1.6 mL PBSM (PBS with 1 mM MgCl2)
Length
69
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
fCpfGpfApfCpUfGpfCpfApfGpfApfGpfCpUUfGpfCpUfApfCpfGpUfGpfApUfCpfApfApfApUfCpfGpfApfGpfCpUUUfApfApfCpfCpfCpfApfApfCpfApfGpfApfGpfCpfApUfCpfCpfGpUfApUfCpUfApUfCpfCpfApfApfApUfGpfGpfGpUfApfCpfCpfGpfApfGpfCpUfCpfGpfApfApUUfCpfCpfCp
Molecular Weight: 22454.14
Extinction Coefficient: 674000
Binding Conditions / Buffer
23°C
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Barfod, Anders et al. "In Vitro Selection of RNA Aptamers Directed Against Protein E: A Haemophilus influenzae Adhesin." Mol Biotech, 2014: 1-12.
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