Clon-3 Aptamer Details
ID# 8193
Structure
Chemistry
DNA
Target
PD-L1
Category
Protein
Affinity (Kd)
70.1 ± 14.2 nM nM
Length
60
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdCpdCpdTpdCpdCpdTpdCpdCpdTpdApdApdCpdTpdGpdTpdTpdCpdCpdTpdApdCpdGpdApdApdApdCpdGpdApdGpdCpdTpdTpdApdTpdGpdCpdGpdTpdApdApdTpdGpdApdTpdGpdApdCpdTpdGpdTpdCpdGpdTpdApdGpdTpdTpdCpdGp-3'
Molecular Weight: 18462
Extinction Coefficient: 640.4
Binding Conditions / Buffer
Binding buffer:
50 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES),
100 mM NaCl, 1 mM MgCl2, 5 mM KCl, and 1 mM CaCl2, pH =
7.5
Binding/Wash buffer (Capture SELEX):
10 mM Tris, 1 mM EDTA,
1 M NaCl, pH 7.4
Varying incubation times:
10min - 1hr
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Li, J., Ren, X., Zhao, J., & Lou, X. (2021). PD-L1 aptamer isolation via Modular-SELEX and its applications in cancer cell detection and tumor tissue section imaging. The Analyst, 146(9), 2910–2918. https://doi.org/10.1039/d1an00182e
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
