Cholic Acid (9) Aptamer Details
ID# 7505
Structure
Chemistry
DNA
Target
Cholic Acid
Category
Small Organic
Affinity (Kd)
50 pM
Length
100
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdApdCpdCpdGpdCpdGpdApdApdGpdApdApdGpdTpdGpdTpdCpdApdTpdTpdGpdTpdTpdTpdTpdGpdGpdApdGpdApdTpdTpdCpdGpdApdApdGpdCpdGpdCpdTpdGpdTpdApdCpdApdCpdApdGpdGpdTpdApdApdTpdGpdApdApdGpdCpdCpdTpdTpdCpdTpdApdApdGpdApdTpdApdGpdTpdApdTpdGpdTpdTpdCpdApdTpdCpdApdGp-3'
Molecular Weight: 30924.2
Extinction Coefficient: 985700
Binding Conditions / Buffer
selection buffer (50 mM Trisƒ??HCl, 300 mM NaCl, 30 mM KCl, 5 mM MgCl2, pH 7.6
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kato, T., et al. "In vitro selection of DNA aptamers which bind to cholic acid." Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1493 (2000):
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