Chloramphenicol (Cm1) Aptamer Details
ID# 7556
Structure
Chemistry
RNA
Target
Chloramphenicol
Category
Small Organic
Affinity (Kd)
110 nM
Length
50
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprUprCprAprCprAprGprUprGprAprAprAprAprAprAprGprAprCprGprUprGprUprGprAprAprUprGprUprCprAprCprAprCprUprGprAprAprAprAprAprAprGprAprUprCprCprCp-3'
Molecular Weight: 16189.95
Extinction Coefficient: 522900
Binding Conditions / Buffer
Binding Buffer (20 mM MgCl2, 400 mM NaCl, 100 mM bis-Tris (pH 6.4)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Burke et al. "RNA aptamers to the peptidyl transferase inhibitor chloramphenicol." Chemistry & Biology, 4(1997): 833-843.
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