CH1S-3 Aptamer Details
ID# 8122
Structure
Chemistry
DNA
Target
Trastuzumab
Category
Antibody
Affinity (Kd)
10.3 nM
Length
28
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdTpdCpdCpdApdGpdGpdGpdTpdTpdCpdCpdApdApdGpdGpdTpdGpdCpdTpdTpdCpdGpdTpdGpdGpdApdCp-3'
Molecular Weight: 9030
Extinction Coefficient: 262400
Binding Conditions / Buffer
Binding buffer was prepared by adding 1 mg/mL BSA and 0.1 mg/mL yeast tRNA into the Dulbecco's PBS (DPBS) containing 0.55 mM MgCl2.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Chen, K.; Zhou, J.; Shao, Z.; Liu, J.; Song, J.; Wang, R.; Li, J.; Tan, W. Aptamers as Versatile Molecular Tools for Antibody Production Monitoring and Quality Control. J. Am. Chem. Soc. 2020 Jun 9; 142(28): 12079-12086. PMID 32516525. DOI: 10.1021/jacs.9b13370.
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