CD63-1 Aptamer Details
ID# 8097
Structure
Chemistry
DNA
Target
CD63
Category
Protein
Affinity (Kd)
38.71 nM
Length
42
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dTpdApdApdCpdApdCpdGpdApdCpdApdGpdApdCpdGpdTpdTpdCpdGpdGpdApdGpdGpdTpdCpdGpdApdApdCpdCpdCpdTpdGpdApdCpdApdGpdCpdGpdTpdGpdGpdGp -3’
Molecular Weight: 12998.4
Extinction Coefficient: 411800
Binding Conditions / Buffer
pH 7.4 PBS containing 2.0 mM MgCl2, 0.02% Tween 20, and 100 µg/mL
Salmon Sperm DNA
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Song Z, Mao J, Barrero RA, Wang P, Zhang F, Wang T. Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation. Molecules. 2020 Nov 27;25(23):5585. doi: 10.3390/molecules25235585. PMID: 33261145; PMCID: PMC7730289.
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