CD4 (Aptamer 7) Aptamer Details
ID# 7526
Structure
Chemistry
2'-F-RNA
Target
CD4
Category
Protein
Affinity (Kd)
0.5 nM
Length
87
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprApfCprAprAprGprAprApfUprAprAprApfCprGpfCpfUpfCprAprAp fUprGpfUprGpfUpfCprGpfUpfCpfCpfUprGprGpfUprApfCprGprApfUpfUpfUpfUprGprGpfUprApfUprApfUprAprApfCpfCprGpfUprGprGpfCpfUpfUp fUpfUpfCprGprApfCprAprGprGprAprGprGpfCpfUpfCprApfCprAprApfCprAprGprGpfCp -3'
Molecular Weight: 28124.72
Extinction Coefficient: 875000
Binding Conditions / Buffer
Elution Buffer: (50 mM sodium phosphate, 150 mM NaCl, 2 M urea and 0.1% NaN3 (pH 7.2)) Binding Binding: (1 ml PBS containing 2 mM MgCl2)
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Davis, K., et al. "Staining of cell surface human CD4 with 2'-F-pyrimidine-containing RNA aptamers for flow cytometry." Nucleic Acids Research, 26(1998): 3915-3924.
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