CD4 (aptamer 12) Aptamer Details

ID# 7735

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Structure

Chemistry

2'-F-RNA

Target

CD4 protein found on CD4+ T-Cells

Sequence (3-char table code)

G A C U/T

5'- rGprGprGprAprGprApfCprAprAprGp rAprApfUprAprAprApfCprGpfCpfUp fCprAprAprGpfUprGprApfCprGpfUp fCpfCpfUprGprApfUpfCprGprApfUp fUprGpfUprGpfCprApfUpfUpfCprGp rGpfUprGpfUprGprApfCprGprApfUp fCpfUpfUpfUpfCprGprApfCprAprGp rGprAprGprGpfCpfUpfCprApfCprAp rApfCprAprGprGpfCp-3'

Molecular Weight: 27852.63

Extinction Coefficient: 861400

Binding Conditions / Buffer

Binding Buffer: PBS containing 1 mM MgCl2

Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

K A Davis et al. "Staining of cell surface human CD4 with 2'-F-pyrimidine-containing RNA aptamers for flow cytometry." Nucleic Acids Res 26(1998):3915-3924.

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