CCRF-CEM (sgc8c) Aptamer Details

ID# 7813

Back to Search Results

Structure

Chemistry

DNA

Target

Acute lymphoblastic leukemia (CCRF-CEM)

Sequence (3-char table code)

G A C U/T

5'-dApdTpdCpdTpdApdApdCpdTpdGpdCpdTpdGpdCpdGpdCpdCpdGpdCpdCpdGpdGpdGpdApdApdApdApdTpdApdCpdTpdGpdTpdApdCpdGpdGpdTpdTpdApdGp-3'

Molecular Weight: 12634.26

Extinction Coefficient: 397600

Binding Conditions / Buffer

Binding buffer: 0.1mg/ml yeast tRNA and 1 mg/ml BSA into wash buffer (4.5g/L glucose, 5 mM MgCl2 in Dulbecco's PBS with CaCl2 and MgCl2)

Binding Temp: 4°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Sequence: Shangguan et al. "Optimization and Modifications of Aptamers Selected from Live Cancer Cell Lines." Chem. Biochem., 8, (2007): 603-6. Doxorubicin conjugation: Y-F Huang et al. Molecular assembly of an aptamer-drug conjugate for targeted drug delivery to tumor cells. Chembiochem. 10(2009):862-868.

Request a Quote Have your aptamer oligo synthesized by Aptagen.