CBSA-5335 SF Aptamer Details
ID# 8120
Structure
Chemistry
DNA
Target
Cocaine
Category
Small Organic
Affinity (Kd)
36 nM
Length
19
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdApdGpdApdCpdApdApdGpdGpXlkdGpdApdCpdApdApdGpdGpdApdGp-3'
Molecular Weight: 5793.8
Extinction Coefficient: 198200
Binding Conditions / Buffer
10% saliva in 10 mM Tris, 0.1 mM MgCl2, pH 7.4
Binding Temp: 23°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yu, H., Canoura, J., Guntupalli, B., Lou, X., & Xiao, Y. (2017). A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva. Chemical Science, 8(1), 131–141. doi:10.1039/c6sc01833e
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