CAP/CAM Aptamer Aptamer Details

ID# 7981

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Structure

Chemistry

RNA

Target

Chloramphenicol

Sequence (3-char table code)

G A C U/T

5'-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdApdCpdTpdTpdCpdApdGpdTpdGpdApdGpdTpdTpdGpdTpdCpdCpdCpdApdCpdGpdGpdTpdCpdGpdGpdCpdGpdApdGpdTpdCpdGpdGpdTpdGpdGpdTpdApdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdCpdGpdTpdGpdApdAp-3'

Molecular Weight: 25106.28

Extinction Coefficient: 781800

Binding Conditions / Buffer

100 mM NaCl, 20 mM Trisƒ??HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6

Binding Temp: 23°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Mehta J, Van Dorst B, Rouah-Martin E, et al. (2010) In vitro selection and characterication of DNA aptamers recognizing chloramphenicol. Journal of Biotechnology 155: 361-369.

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