cAMP Aptamer Details
ID# 7512
Structure
Chemistry
RNA
Target
cAMP
Category
Small Organic
Affinity (Kd)
10 µM
Length
31
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-{5'/Phosphate}-rGprGprAprAprGprAprGprAprUprGprGprCprGprAprCprUprAprAprAprAprCprGprAprCprUprUprGprUprCprGprCp-3'
Molecular Weight: 10044.17
Extinction Coefficient: 316000
Binding Conditions / Buffer
Binding Buffer (20 mM Tris-HCl (pH 7.5 at 23 °C), 450 mM NaCl, 100 mM KCl, 10 mM MgCl2, 1 mM MnCl2, and 5 mM CaCl2)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kozumi and Breaker. "Molecular Recognition of cAMP by an RNA Aptamer." Biochemistry, 39 (2000): 8983-8992.
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