Caffeine (S2.caf.D11) Aptamer Details
ID# 7651
Structure
Chemistry
RNA
Target
Caffeine
Category
Protein
Affinity (Kd)
1mM mM
Length
99
Aptazyme
Yes
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprAprUprGprUprCprCprAprGprUprCprGprCprUprUprGprCprAprAprUprGprCprCprCprUprUprUprUprAprGprAprCprCprCprUprGprAprUprGprAprGprGprAprUprCprAprUprCprGprGprAprCprUprUprUprGprUprCprCprUprGprUprGprGprAprGprUprAprAprGprAprUprCprGprCprGprAprAprAprCprGprGprUprGprAprAprAprGprCprCprGprUprAprGprGprUprCprUp-3'
Molecular Weight: 31866.13
Extinction Coefficient: 976100
Binding Conditions / Buffer
Selection Buffer (500 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 10 mM sodium phosphate, 90 mM HEPES (pH 7.5)) Assay Buffer (180mM HEPES (pH 7.0?ñ8.0), 300 mM NaCl, 20 or 50 mM MgCl2, 2 mM EDTA, 20 mM Na phosphate), r.t.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ferguson et al. "A novel strategy for selection of allosteric ribozymes yields RiboReporter TM sensors for caffeine and aspartame." Nucleic Acids Research, 32(2004): 1756-1766.
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