C5s Anti-Antibody Aptamer Details

ID# 7959

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Structure

Chemistry

DNA

Target

Anti-Vesicular Stomatitis Antibody

Sequence (3-char table code)

G A C U/T

5'-dApdCpdTpdApdGpdGpdApdCpdGpdCpdTpdTpdGpdGpdGpdApdGpdGpdGpdGpdGpdGpdGpdTpdGpdGpdGpdGpdTpdGpdTpdCpdCpdGpdGpdTpdCpdGpdCpdGp-3'

Molecular Weight: 12587.16

Extinction Coefficient: 385000

Binding Conditions / Buffer

90% High Glucose DMEM, 10% Fetal Bovine Serum. Tested in vivo.

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Muharemagic D, Zamay A, Ghobadloo SM, Evgin L, Savitskaya A, et al. (2014) Aptamer-Facilitated Protection of Oncolytic Virus from Neutralizing Antibodies. Molecular Therapy--Nucleic Acids 3, e167. doi: 10.1038/mtna.2014.19

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